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Above- and belowground biomass and nutrient pools of Spartina alterniflora (smooth cordgrass) in a South American salt marsh Author: Negrin, Vanesa L., de Villalobos, Ana E., Trilla, Gabriela González, Botté, Sandra E., Marcovecchio, Jorge E. h�bbd```b``� "&�H�� ��̞"Y��9 �� Additionally, fingerprinting and field evaluation of a sample of 44 clones collected from brown marsh areas are under progress. Late flowering accessions were associated with increased spread and tiller density but not with the other traits. Louisiana Agriculture 46 (4):42-44. Data Source and References for Spartina alterniflora (smooth cordgrass) from the USDA PLANTS database Name Search name search type enter a search name In case of RAPD, 176 fragments were polymorphic where as 708 fragments were polymorphic in AFLP analysis. Smooth cordgrass will tolerate regular inundations with 0 to 35 parts per thousand salinity. Analysis of another set of 38 S. alterniflora plants collected from brown marsh areas using AFLP technique indicated existence of unique genetic variation which will be useful in breeding superior plants with tolerance to abiotic stresses. Significant progress has been made during the first year of the initiation of this project. DNA fingerprinting protocols developed through this project will help to test the authenticity of plant materials used in the restoration projects. J. Spartina has infested Willapa Bay, Washington, and other natural settings and done great damage to the natural marine environment. Plant and Animal Genome XI, San Diego, CA, Jan 11-15, 2003, Final Abstract guide, p:113. Development of smooth cordgrass lines with improved seed producing ability and other desirable growth characteristics and stress tolerance will accelerate the on-going restoration projects by providing options for a range of improved plant materials, thus directly impacting the economy and environment of the state of Louisiana.Publications, Progress 01/01/03 to 12/31/03OutputsThe overall goal of this project is to develop improved smooth cordgrass lines for utilization in coastal restoration projects. h�b```a``�"e�@����(����3 � (����� �+�� -�"`�?Y�2�a^���\�����Μq/E���J:Q�I����lL��4#��fN&{)�(S@� ~�n Plant Sci. The mean percent germination was 43 % with a range of 3 to 89%. 72(1): 138-148. Ecology. Development of smooth cordgrass lines with improved seed producing ability and other desirable characteristics will accelerate the on-going restoration projects by providing options for a range of improved plant materials.Publications, Progress 01/01/02 to 12/31/02OutputsCoastal erosion and wetland deterioration are serious and widespread in the state of Louisiana. (smooth cordgrass) (Denno 1977, 1978), with Spartina alterniflora “…an inferior host plant for development” (Denno 1977: 366). Progress made during the reporting period include completion of the phenotypic and molecular characterization of 40 selected lines from the collected germplasm and 38 lines from brown marsh sites, selection of superior clones from the mutagenized population and polymorphism survey of the parents for construction of the Spartina molecular map. Considerations for Large Scale Collection of Smooth Cordgrass Seed (Spartina alterniflora.L) William Skaradek, and Noel Murray USDA-NRCS Cape May Plant Materials Center and … Protocols for fingerprinting Spartina Progress made by the end of this project includes completion of the phenotypic and molecular characterization of S. alterniflora lines, selection of superior lines and polymorphism survey of the parents for construction of the Spartina molecular map. In addition, development of smooth cordgrass lines with improved seed producing ability and other desirable characteristics also will accelerate the on-going restoration projects by providing options for a range of improved plant materials, thus directly impacting the economy and environment of the state of Louisiana.Publications, National Institute of Food and Agriculture, 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants, 212 - Pathogens and Nematodes Affecting Plants, 201 - Plant Genome, Genetics, and Genetic Mechanisms. Rescuing the coast with biotechnology. Smooth cordgrass (Spartina alterniflora) is used extensively for shoreline protection and tidal marsh restoration on the Atlantic and Gulf coasts because of its aggressive colonizing ability and tolerance to a wide range of salinity. Spartina brasiliensis Raddi Spartina glabra Muhl. 154 0 obj <>stream Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. The hybrids were evaluated in the marsh, and the best performing ones were identified for next round of crossing and selection. In the southernmost part of coast of China, two height forms of Spartina alterniflora, tall and short, have invaded Leizhou Peninsula within the last decade. For a current distribution map, please consult the Plant Profile page for this species on the PLANTS Website. A number of crosses have been made for initiating the recurrent selection program. Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. Progress 09/01/01 to 03/31/04OutputsThe overall goal of this project is to apply plant breeding and genetics principles to develop improved smooth cordgrass (Spartina alterniflora) lines for utilization in coastal restoration projects. S. alterniflora plants collected from brown marsh areas indicated existence of unique genetic variation which will be useful in breeding superior plants to resist brown marsh phenomenon. Research Project: Use of Classical and Molecular Technologies for Developing Aflatoxin Resistance in Crops Location: Food and Feed Safety Research Title: An actin-depolymerizing factor from the halophyte smooth cordgrass, Spartina alterniflora (SaADF2), is superior to its rice homolog (OsADF2) in conferring drought and salt tolerance when constitutively overexpressed in rice Distichlis spicata (L.) Greene (saltgrass, AFLP analysis of another set of 38 165(3):395-401. 117 0 obj <>/Filter/FlateDecode/ID[<5AA68FA4C9FF314BB0D91E1A72901282>]/Index[96 59]/Info 95 0 R/Length 108/Prev 148061/Root 97 0 R/Size 155/Type/XRef/W[1 3 1]>>stream Trachynotia alterniflora Steud. The clones are also currently fingerprinted to determine and compare the amount of genetic variation between the clones and seedlings. This plant can be weedy or invasive according to the authoritative sources noted below.This plant may be known by one or more common names in different places, and some are listed above. Invasive Spartina alterniflora (S. alterniflora), a native riparian species in the U.S. Gulf of Mexico, has led to serious degradation to the ecosystem and biodiversity as well as economic losses since it was introduced to China in 1979. Several RAPD primers were used to screen the hybrid mapping population which confirmed the hybrid nature of the population making it suitable for development of a molecular map.ImpactsCostal wetland loss continues to be a major environmental problem in the state of Louisiana. and Harrison, S.A. 2004. 2011). Spartina alterniflora is a perennial grass that expands via underground rhizomes. [6] The hybrid can physically modify the environment to the detriment of native species, [ 7 ] and the hybrid populations have spread into creeks , bays , and more remote coastal locations. Positive correlations were found among all vegetative traits representing plant growth. p:12. Observations were recorded two times on vigor, spread, tillering and rust infection on individual plants in the mutagenized population. Ryan, A.B., Venuto, B., Harrison, S., Subudhi, P., and Materne, M. 2002. Wave attenuation characteristics for rigid and flexible model vegetation as well as live Spartina alterniflora and Juncus roemerianus, two common species along coastal Mississippi and Louisiana, were investigated under both The Jaccard similarity coefficient computed from RAPD and AFLP data ranged from 22% to 89%, indicating a high level of genetic diversity. Spartina alterniflora is an invasive grass that has spread to more than 10,000 acres in Washington state and more than 1,000 acres in San Francisco Bay and tidelands. Conversely, negative correlations between growth traits and rust reaction were observed. Ryan, A.B., Subudhi, P.K., Venuto, B.C., Harrison, S.A., and Shadow, R.A. 2003. marshhay cordgrass Spartina patens (Ait.)Muhl. Spartina alterniflora) in June and the results in October USDA is an equal opportunity provider, employer, and lender. %PDF-1.7 %���� Spartina alterniflora. Positive correlations were found among all vegetative traits representing plant growth. %%EOF common cordgrass Spartina anglica C.E. The differences in clustering pattern indicated the potential of molecular markers in accurate assessment of genetic diversity. Hubbard denseflower cordgrass Spartina densiflora Brongn. DC. Plant and Animal Genome XI, San Diego, CA, Jan 11-15, 2003, Final Abstract guide, p:113. Progress 01/01/02 to 12/31/02 Outputs Activities included collecting 50 Spartina alterniflora accessions from highly impacted dying marsh sites in Louisiana. Bertness, Mark D. 1991. We isolated a v-ATPase gene (SaVHAc1) from Spartina alterniflora or smooth cordgrass, a plant native to Louisiana coastal salt marshes and expressed it in transgenic tobacco and rice. Using RAPD and AFLP markers, 40 genotypes from seven accessions were fingerprinted. Spartina alterniflora, the smooth cordgrass, saltmarsh cordgrass, or salt-water cordgrass, is a perennial deciduous grass which is found in intertidal wetlands, especially estuarine salt marshes. Assessment of genetic diversity in Louisiana native smooth cordgrass (Spartina alterniflora) accessions using molecular markers. Be found growing on sandy aerobic or anaerobic soils with pHs ranging from 3.7 to.... Traits and 45 plants with consistent superior rating were selected for further evaluation results demonstrated a improvement... Usda NRCS Golden Meadows PMC ( LAPMC ) the USDA, natural Resources Conservation Service ( NRCS ) Golden. Conversely, negative correlations between growth traits and rust infection on individual.... Preserving, protecting and defending coastal Louisiana selected S. alterniflora into a biological resource to inhibit M. )... The differences in clustering pattern in both studies indicated that the use of molecular markers XI, San Diego CA... Grande Terre, Louisiana, showed that seed set at Baton Rouge Grande... Ranged from 0 to 94 % ‘ Vermilion ’ smooth cordgrass ( Spartina alterniflora on interaction... B., Harrison, S.A. and Shadow, R.A. 2003 local ecological environment the first year of the seed germination., protecting and defending coastal Louisiana live Spartina alterniflora ) accessions using molecular markers and tiller density but with... At Baton Rouge was 47 % and ranged from 0 to 94 % Activities included collecting Spartina. Of variability was noted for these traits and 45 plants with consistent rating. Restored marshlands can help slow down the coastal wetland loss genotypes from seven accessions were subjected to both field and!, P., and other physiological adaptations such as reduced stomatal density and closure of stomata to overcome stress S.. 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